Detailed information of TA system TA048192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4720974..4721576
• Replicon: chromosome
• Accession: NZ_CP009050
• Organism: Escherichia coli NCCP15648

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A610_RS25020
• Protein ID: WP_000897305.1
• Coordinates: 4720974..4721285 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A610_RS25025
• Protein ID: WP_000356397.1
• Coordinates: 4721286..4721576 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A610_RS24995	4716888..4717487	+	600	WP_001295269.1	glucose-1-phosphatase	-
A610_RS25000	4717481..4718353	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A610_RS25005	4718350..4718787	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
A610_RS25010	4718832..4719773	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A610_RS25015	4719837..4720745	-	909	WP_001162704.1	alpha/beta hydrolase	-
A610_RS25020	4720974..4721285	+	312	WP_000897305.1	hypothetical protein	Toxin
A610_RS25025	4721286..4721576	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
A610_RS25035	4722162..4722380	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
A610_RS25040	4722600..4722842	+	243	WP_001087409.1	hypothetical protein	-
A610_RS25045	4723172..4724101	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
A610_RS25050	4724098..4724733	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A610_RS25055	4724730..4725632	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T48192 WP_000897305.1 NZ_CP009050:4720974-4721285 [Escherichia coli NCCP15648]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T48192 NZ_CP009050:4720974-4721285 [Escherichia coli NCCP15648]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT48192 WP_000356397.1 NZ_CP009050:4721286-4721576 [Escherichia coli NCCP15648]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT48192 NZ_CP009050:4721286-4721576 [Escherichia coli NCCP15648]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7