Detailed information of TA system TA048191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3894417..3895216
• Replicon: chromosome
• Accession: NZ_CP009050
• Organism: Escherichia coli NCCP15648

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: A610_RS20775
• Protein ID: WP_000347267.1
• Coordinates: 3894752..3895216 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: A610_RS20770
• Protein ID: WP_001307405.1
• Coordinates: 3894417..3894752 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A610_RS20755	3890203..3890973	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
A610_RS20760	3890989..3892323	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
A610_RS20765	3892697..3894268	+	1572	WP_001273741.1	galactarate dehydratase	-
A610_RS20770	3894417..3894752	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
A610_RS20775	3894752..3895216	+	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
A610_RS20780	3895271..3896080	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
A610_RS20790	3896329..3897609	+	1281	WP_000681935.1	tagatose-bisphosphate aldolase subunit KbaZ	-
A610_RS20795	3897632..3898105	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
A610_RS20800	3898116..3898895	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
A610_RS20805	3898885..3899763	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
A610_RS20810	3899781..3900215	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3882151..3895216	13065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T48191 WP_000347267.1 NZ_CP009050:3894752-3895216 [Escherichia coli NCCP15648]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T48191 NZ_CP009050:3894752-3895216 [Escherichia coli NCCP15648]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT48191 WP_001307405.1 NZ_CP009050:3894417-3894752 [Escherichia coli NCCP15648]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT48191 NZ_CP009050:3894417-3894752 [Escherichia coli NCCP15648]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7