Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2581581..2582083 Replicon chromosome
Accession NZ_CP009050
Organism Escherichia coli NCCP15648

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A610_RS14050 Protein ID WP_000767829.1
Coordinates 2581581..2581835 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A610_RS14055 Protein ID WP_001259255.1
Coordinates 2581832..2582083 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A610_RS14020 2576596..2576823 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
A610_RS14025 2576837..2577895 - 1059 WP_000492324.1 transport protein YeeE -
A610_RS14030 2578074..2579432 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A610_RS14035 2579422..2579484 - 63 WP_010723108.1 membrane protein YoeI -
A610_RS14040 2579699..2580628 - 930 WP_000803366.1 LysR family transcriptional regulator -
A610_RS14045 2580674..2581498 - 825 WP_000754758.1 SDR family oxidoreductase -
A610_RS14050 2581581..2581835 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A610_RS14055 2581832..2582083 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A610_RS14060 2582367..2582417 + 51 WP_001364200.1 his operon leader peptide -
A610_RS14070 2582563..2583462 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
A610_RS14075 2583468..2584772 + 1305 WP_001386882.1 histidinol dehydrogenase -
A610_RS14080 2584769..2585839 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
A610_RS14085 2585839..2586906 + 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T48186 WP_000767829.1 NZ_CP009050:c2581835-2581581 [Escherichia coli NCCP15648]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T48186 NZ_CP009050:c2581835-2581581 [Escherichia coli NCCP15648]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT48186 WP_001259255.1 NZ_CP009050:c2582083-2581832 [Escherichia coli NCCP15648]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT48186 NZ_CP009050:c2582083-2581832 [Escherichia coli NCCP15648]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References