Detailed information of TA system TA048172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4746613..4747294
• Replicon: chromosome
• Accession: NZ_CP009048
• Organism: Pseudomonas alkylphenolica strain KL28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PSAKL28_RS21750
• Protein ID: WP_038616925.1
• Coordinates: 4746926..4747294 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A077FDE4
• Locus tag: PSAKL28_RS21745
• Protein ID: WP_038614479.1
• Coordinates: 4746613..4746942 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSAKL28_RS21725	4741951..4743357	+	1407	WP_038614471.1	FAD-binding oxidoreductase	-
PSAKL28_RS21730	4743390..4743731	+	342	WP_038614473.1	cupin domain-containing protein	-
PSAKL28_RS21735	4743786..4744862	+	1077	WP_038614475.1	polyamine ABC transporter substrate-binding protein	-
PSAKL28_RS21740	4744924..4746507	-	1584	WP_038614477.1	peptide chain release factor 3	-
PSAKL28_RS21745	4746613..4746942	-	330	WP_038614479.1	XRE family transcriptional regulator	Antitoxin
PSAKL28_RS21750	4746926..4747294	-	369	WP_038616925.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSAKL28_RS21755	4747731..4748417	+	687	WP_167335153.1	ABC transporter permease	-
PSAKL28_RS21760	4748432..4749340	+	909	WP_038614484.1	glycine betaine ABC transporter substrate-binding protein	-
PSAKL28_RS21765	4749337..4749990	+	654	WP_038614486.1	ABC transporter permease	-
PSAKL28_RS21770	4749987..4751141	+	1155	WP_038614488.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (10-87)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14233.08 Da
• Isoelectric Point: 7.2452

>T48172 WP_038616925.1 NZ_CP009048:c4747294-4746926 [Pseudomonas alkylphenolica]
MPWSIRFCTEFVSDFEDLTEQIQDELLSQLRVLESRGPALGRPRVDTLKGSRYHNMKELRFDAQNGVWRVAFAFDPKRCA
IILVAGDKAGVSQQRFYRNLIRHADERFERYLSGGEEHVQNT

Nucleotide

• Length: 369 bp

>T48172 NZ_CP009048:c4747294-4746926 [Pseudomonas alkylphenolica]
TTGCCATGGTCAATCAGGTTTTGTACTGAGTTTGTTTCGGACTTCGAGGATTTGACTGAACAGATCCAGGATGAGTTACT
CAGCCAGCTGAGGGTGCTGGAGAGCCGCGGACCAGCACTTGGGCGGCCGCGCGTGGATACGTTGAAAGGATCTCGATATC
ACAACATGAAGGAGCTGCGGTTTGACGCTCAAAATGGTGTCTGGCGAGTGGCTTTTGCATTTGATCCCAAGCGATGCGCC
ATTATTCTCGTGGCAGGCGACAAGGCTGGAGTGAGCCAGCAACGGTTTTACCGGAATCTGATCCGGCATGCCGATGAGCG
TTTTGAAAGGTATTTGTCAGGAGGTGAGGAACATGTCCAAAACACTTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12263.05 Da
• Isoelectric Point: 7.2076

>AT48172 WP_038614479.1 NZ_CP009048:c4746942-4746613 [Pseudomonas alkylphenolica]
MSKTLDEVINSLSPERRARIEQRGRELIREEMTLQALRKQLELTQEGMAERLDIRQANISKVERRSDMLISTLRTYVEAM
GGTLELVAHMPGRGPVTLEGFADGSGGHK

Nucleotide

• Length: 330 bp

>AT48172 NZ_CP009048:c4746942-4746613 [Pseudomonas alkylphenolica]
ATGTCCAAAACACTTGATGAAGTCATCAACAGTCTTTCGCCCGAACGCCGGGCACGCATCGAGCAACGCGGGCGTGAGCT
GATTCGCGAAGAAATGACCTTGCAGGCATTGCGCAAACAGCTGGAGCTTACCCAGGAAGGCATGGCCGAGCGTCTGGACA
TCCGTCAGGCGAATATTTCCAAGGTCGAGAGGCGCAGCGACATGCTGATTTCGACCCTGCGCACCTACGTGGAAGCAATG
GGCGGAACACTTGAACTGGTTGCACATATGCCGGGTCGCGGGCCTGTCACCCTTGAAGGTTTCGCTGACGGCTCGGGAGG
ACATAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A077FDE4