Detailed information of TA system TA047999

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ldrD-agrB/Ldr(toxin)
• Location: 4529750..4529971
• Replicon: chromosome
• Accession: NZ_CP008957
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: ldrD
• Uniprot ID: S1NWX0
• Locus tag: EDL933_RS32825
• Protein ID: WP_001295224.1
• Coordinates: 4529750..4529857 (-)
• Length: 36 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4529906..4529971 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDL933_RS23545	4525003..4525755	-	753	Protein_4453	cellulose biosynthesis protein BcsQ	-
EDL933_RS23555	4525767..4525955	-	189	WP_001063316.1	YhjR family protein	-
EDL933_RS23560	4526228..4527799	+	1572	WP_001204920.1	cellulose biosynthesis protein BcsE	-
EDL933_RS23565	4527796..4527987	+	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
EDL933_RS23570	4527984..4529663	+	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
EDL933_RS32825	4529750..4529857	-	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	Toxin
-	4529906..4529971	+	66	NuclAT_17	-	Antitoxin
-	4529906..4529971	+	66	NuclAT_17	-	Antitoxin
-	4529906..4529971	+	66	NuclAT_17	-	Antitoxin
-	4529906..4529971	+	66	NuclAT_17	-	Antitoxin
-	4529906..4529971	+	66	NuclAT_22	-	Antitoxin
-	4529906..4529971	+	66	NuclAT_22	-	Antitoxin
-	4529906..4529971	+	66	NuclAT_22	-	Antitoxin
-	4529906..4529971	+	66	NuclAT_22	-	Antitoxin
EDL933_RS23585	4530333..4531604	+	1272	WP_001301684.1	amino acid permease	-
EDL933_RS23590	4531634..4532638	-	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
EDL933_RS23595	4532635..4533618	-	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	-
EDL933_RS23600	4533629..4534531	-	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 36 a.a.
• Molecular weight: 3882.70 Da
• Isoelectric Point: 9.0157

>T47999 WP_001295224.1 NZ_CP008957:c4529857-4529750 [Escherichia coli O157:H7 str. EDL933]
MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK

Nucleotide

• Length: 108 bp

>T47999 NZ_CP008957:c4529857-4529750 [Escherichia coli O157:H7 str. EDL933]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT47999 NZ_CP008957:4529906-4529971 [Escherichia coli O157:H7 str. EDL933]
GTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A4P7TSJ7

Antitoxin