Detailed information of TA system TA047994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4103864..4104663
• Replicon: chromosome
• Accession: NZ_CP008957
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: EDL933_RS21360
• Protein ID: WP_000347273.1
• Coordinates: 4104199..4104663 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: EDL933_RS21355
• Protein ID: WP_001302819.1
• Coordinates: 4103864..4104199 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDL933_RS21340	4099649..4100419	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
EDL933_RS21345	4100435..4101769	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
EDL933_RS21350	4102144..4103715	+	1572	WP_001273776.1	galactarate dehydratase	-
EDL933_RS21355	4103864..4104199	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
EDL933_RS21360	4104199..4104663	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
EDL933_RS21365	4104718..4105527	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
EDL933_RS21370	4105776..4107056	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
EDL933_RS21375	4107079..4107552	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
EDL933_RS21380	4107563..4108342	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
EDL933_RS21385	4108332..4109210	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
EDL933_RS21390	4109228..4109662	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T47994 WP_000347273.1 NZ_CP008957:4104199-4104663 [Escherichia coli O157:H7 str. EDL933]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T47994 NZ_CP008957:4104199-4104663 [Escherichia coli O157:H7 str. EDL933]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT47994 WP_001302819.1 NZ_CP008957:4103864-4104199 [Escherichia coli O157:H7 str. EDL933]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT47994 NZ_CP008957:4103864-4104199 [Escherichia coli O157:H7 str. EDL933]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7