Detailed information of TA system TA047977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 289892..290586
• Replicon: chromosome
• Accession: NZ_CP008957
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: EDL933_RS01360
• Protein ID: WP_001263495.1
• Coordinates: 290188..290586 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: EDL933_RS01355
• Protein ID: WP_000554757.1
• Coordinates: 289892..290185 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDL933_RS33000	285603..286100	+	498	Protein_249	REP-associated tyrosine transposase RayT	-
EDL933_RS01340	286245..287957	-	1713	Protein_250	flagellar biosynthesis protein FlhA	-
EDL933_RS01345	287929..288714	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
EDL933_RS01350	288785..289840	+	1056	WP_001226188.1	DNA polymerase IV	-
EDL933_RS01355	289892..290185	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EDL933_RS01360	290188..290586	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EDL933_RS01365	290596..291048	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
EDL933_RS01370	291367..291570	+	204	Protein_256	RNA ligase RtcB family protein	-
EDL933_RS01375	291569..292090	+	522	Protein_257	peptide chain release factor H	-
EDL933_RS01380	292147..293604	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
EDL933_RS01385	293865..294323	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	294919..294999	+	81	NuclAT_12	-	-
-	294919..294999	+	81	NuclAT_12	-	-
-	294919..294999	+	81	NuclAT_12	-	-
-	294919..294999	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T47977 WP_001263495.1 NZ_CP008957:290188-290586 [Escherichia coli O157:H7 str. EDL933]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T47977 NZ_CP008957:290188-290586 [Escherichia coli O157:H7 str. EDL933]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT47977 WP_000554757.1 NZ_CP008957:289892-290185 [Escherichia coli O157:H7 str. EDL933]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT47977 NZ_CP008957:289892-290185 [Escherichia coli O157:H7 str. EDL933]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6