Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-MqsA
Location 288998..289575 Replicon chromosome
Accession NZ_CP008941
Organism Candidatus Paracaedibacter acanthamoebae isolate PRA3

Toxin (Protein)


Gene name higB Uniprot ID A0A077ATD2
Locus tag ID47_RS01135 Protein ID WP_038462941.1
Coordinates 288998..289306 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A077AR43
Locus tag ID47_RS01140 Protein ID WP_038462944.1
Coordinates 289303..289575 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID47_RS01120 284022..284369 - 348 WP_038462935.1 hypothetical protein -
ID47_RS01125 284489..286279 + 1791 WP_051908370.1 type IV secretion system DNA-binding domain-containing protein -
ID47_RS01130 286589..288754 + 2166 WP_038462938.1 ATP-dependent RecD-like DNA helicase -
ID47_RS01135 288998..289306 + 309 WP_038462941.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ID47_RS01140 289303..289575 + 273 WP_038462944.1 helix-turn-helix domain-containing protein Antitoxin
ID47_RS01145 289870..290358 - 489 WP_038462946.1 hypothetical protein -
ID47_RS01150 290550..291131 - 582 WP_038462949.1 mobile mystery protein B -
ID47_RS01155 291128..291601 - 474 WP_051908373.1 mobile mystery protein A -
ID47_RS01160 292020..292697 - 678 WP_038462951.1 IS6 family transposase -
ID47_RS01165 292769..292972 - 204 Protein_235 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-97)

Antitoxin

Antitoxin MqsA/HigA-2

(24-86)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11760.60 Da        Isoelectric Point: 9.6241

>T47951 WP_038462941.1 NZ_CP008941:288998-289306 [Candidatus Paracaedibacter acanthamoebae]
MEFIESPLFSKLISSYLDNDEYAAFQWDLALHPEKGDIIPSSGGLRKIRWSAKGKGKRGGVRVIYFYQNKQGQIWLLTIY
AKNEDENIPLSVLKKVKKELEI

Download         Length: 309 bp

>T47951 NZ_CP008941:288998-289306 [Candidatus Paracaedibacter acanthamoebae]
ATGGAATTTATAGAATCACCTTTATTCAGTAAGCTTATTTCATCTTATCTTGATAATGATGAGTATGCGGCTTTTCAATG
GGACTTAGCCCTACATCCTGAAAAAGGAGACATCATTCCAAGCAGCGGTGGATTAAGGAAAATTAGATGGTCAGCAAAAG
GTAAAGGAAAACGGGGAGGGGTAAGAGTTATATACTTTTACCAGAATAAACAAGGGCAAATTTGGTTACTGACTATTTAT
GCTAAAAATGAGGATGAAAATATCCCCTTATCCGTATTGAAAAAGGTTAAAAAGGAGTTAGAAATATGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10113.66 Da        Isoelectric Point: 10.2744

>AT47951 WP_038462944.1 NZ_CP008941:289303-289575 [Candidatus Paracaedibacter acanthamoebae]
MNNSRNIGQEILEALNEIKNGKGVIKKIDACKDVSRIRQELNLSQAAFSSLMGISVRTLQEWEQGRRYPTGPALSLLRIA
HKHPEAFVNL

Download         Length: 273 bp

>AT47951 NZ_CP008941:289303-289575 [Candidatus Paracaedibacter acanthamoebae]
ATGAATAATTCTAGAAATATTGGTCAGGAAATTTTAGAAGCTCTTAATGAAATAAAAAATGGCAAAGGTGTCATAAAAAA
GATAGATGCTTGCAAAGATGTTAGCCGTATACGACAGGAATTAAATCTTTCCCAAGCCGCCTTTTCTTCGCTTATGGGGA
TTAGTGTGCGTACTTTACAAGAGTGGGAACAAGGAAGACGGTATCCTACAGGACCAGCTTTATCCTTATTAAGGATTGCC
CATAAACATCCTGAAGCATTTGTCAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A077ATD2

Antitoxin

Source ID Structure
AlphaFold DB A0A077AR43

References