Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3124040..3124637 Replicon chromosome
Accession NZ_CP008929
Organism Klebsiella pneumoniae strain PMK1

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag PMK1_RS15185 Protein ID WP_004142563.1
Coordinates 3124040..3124357 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag PMK1_RS15190 Protein ID WP_004142561.1
Coordinates 3124350..3124637 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PMK1_RS15170 3119740..3121167 - 1428 WP_009308097.1 MFS transporter -
PMK1_RS15175 3121276..3122142 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
PMK1_RS15180 3122812..3123855 + 1044 WP_020325241.1 DUF2157 domain-containing protein -
PMK1_RS15185 3124040..3124357 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PMK1_RS15190 3124350..3124637 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
PMK1_RS15195 3124902..3125555 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
PMK1_RS15200 3125675..3126043 - 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
PMK1_RS15205 3126043..3126624 - 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
PMK1_RS15210 3126804..3127919 + 1116 WP_020325248.1 MBL fold metallo-hydrolase -
PMK1_RS15215 3127950..3128291 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
PMK1_RS15220 3128309..3128557 - 249 WP_020325243.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T47934 WP_004142563.1 NZ_CP008929:3124040-3124357 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T47934 NZ_CP008929:3124040-3124357 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT47934 WP_004142561.1 NZ_CP008929:3124350-3124637 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT47934 NZ_CP008929:3124350-3124637 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References