Detailed information of TA system TA047834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2446879..2447474
• Replicon: chromosome
• Accession: NZ_CP008873
• Organism: Pseudomonas aeruginosa strain F9670

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HW11_RS31580
• Protein ID: WP_003117425.1
• Coordinates: 2446879..2447157 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW11_RS11210
• Protein ID: WP_003099268.1
• Coordinates: 2447169..2447474 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW11_RS11195	2442305..2443543	+	1239	WP_014603324.1	C69 family dipeptidase	-
HW11_RS11200	2443605..2444252	+	648	WP_003095021.1	carbonate dehydratase	-
HW11_RS11205	2444322..2446550	-	2229	WP_003099265.1	TonB-dependent receptor	-
HW11_RS31580	2446879..2447157	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW11_RS11210	2447169..2447474	+	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HW11_RS31585	2447851..2448378	+	528	WP_071535723.1	AAA family ATPase	-
HW11_RS31590	2448390..2449928	-	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
HW11_RS31595	2449903..2450697	-	795	WP_023082695.1	helix-turn-helix domain-containing protein	-
HW11_RS11215	2450871..2451137	-	267	WP_016852153.1	hypothetical protein	-
HW11_RS11220	2451247..2451519	+	273	WP_003115921.1	hypothetical protein	-
HW11_RS11230	2451731..2452021	+	291	WP_023083237.1	DUF5447 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T47834 WP_003117425.1 NZ_CP008873:2446879-2447157 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47834 NZ_CP008873:2446879-2447157 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT47834 WP_003099268.1 NZ_CP008873:2447169-2447474 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47834 NZ_CP008873:2447169-2447474 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5