Detailed information of TA system TA047832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5295103..5295698
• Replicon: chromosome
• Accession: NZ_CP008872
• Organism: Pseudomonas aeruginosa strain X78812

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HV94_RS24880
• Protein ID: WP_003113526.1
• Coordinates: 5295420..5295698 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV94_RS24875
• Protein ID: WP_003099268.1
• Coordinates: 5295103..5295408 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV94_RS24865	5290589..5290924	+	336	WP_031675806.1	hypothetical protein	-
HV94_RS24870	5290980..5292905	-	1926	WP_023085762.1	DUF3732 domain-containing protein	-
HV94_RS29915	5292902..5293369	-	468	WP_124075005.1	hypothetical protein	-
HV94_RS29920	5293366..5294466	-	1101	WP_124075006.1	hypothetical protein	-
HV94_RS24875	5295103..5295408	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HV94_RS24880	5295420..5295698	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV94_RS24890	5296027..5298255	+	2229	WP_023090880.1	TonB-dependent receptor	-
HV94_RS24895	5298325..5298972	-	648	WP_003095021.1	carbonate dehydratase	-
HV94_RS24900	5299034..5300272	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T47832 WP_003113526.1 NZ_CP008872:c5295698-5295420 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47832 NZ_CP008872:c5295698-5295420 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT47832 WP_003099268.1 NZ_CP008872:c5295408-5295103 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47832 NZ_CP008872:c5295408-5295103 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3