Detailed information of TA system TA047819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5760480..5761075
• Replicon: chromosome
• Accession: NZ_CP008870
• Organism: Pseudomonas aeruginosa strain W36662

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HV97_RS27550
• Protein ID: WP_003113526.1
• Coordinates: 5760797..5761075 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV97_RS27545
• Protein ID: WP_003113527.1
• Coordinates: 5760480..5760785 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV97_RS27525	5755954..5756289	+	336	WP_031633682.1	hypothetical protein	-
HV97_RS27530	5756821..5758587	-	1767	WP_023087829.1	hypothetical protein	-
HV97_RS27535	5758765..5759506	-	742	Protein_5308	restriction endonuclease	-
HV97_RS27540	5759730..5760143	-	414	WP_049231965.1	hypothetical protein	-
HV97_RS27545	5760480..5760785	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HV97_RS27550	5760797..5761075	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV97_RS27560	5761404..5763632	+	2229	WP_023109375.1	TonB-dependent receptor	-
HV97_RS27565	5763702..5764349	-	648	WP_003095021.1	carbonate dehydratase	-
HV97_RS27570	5764411..5765649	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T47819 WP_003113526.1 NZ_CP008870:c5761075-5760797 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47819 NZ_CP008870:c5761075-5760797 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT47819 WP_003113527.1 NZ_CP008870:c5760785-5760480 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47819 NZ_CP008870:c5760785-5760480 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3