Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3547677..3548452 Replicon chromosome
Accession NZ_CP008870
Organism Pseudomonas aeruginosa strain W36662

Toxin (Protein)


Gene name yhaV Uniprot ID V6ADY6
Locus tag HV97_RS16680 Protein ID WP_009518525.1
Coordinates 3547677..3548135 (-) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A7M2ZSF3
Locus tag HV97_RS16685 Protein ID WP_023087286.1
Coordinates 3548135..3548452 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV97_RS16655 3543040..3543987 + 948 WP_023087291.1 TIGR03756 family integrating conjugative element protein -
HV97_RS16660 3543997..3545391 + 1395 WP_023087290.1 integrating conjugative element protein -
HV97_RS16665 3545388..3545747 + 360 WP_023087289.1 hypothetical protein -
HV97_RS16670 3545761..3547284 + 1524 WP_023087288.1 conjugal transfer protein TraG N-terminal domain-containing protein -
HV97_RS16675 3547291..3547650 - 360 WP_023087287.1 DUF3742 family protein -
HV97_RS16680 3547677..3548135 - 459 WP_009518525.1 type II toxin-antitoxin system YhaV family toxin Toxin
HV97_RS16685 3548135..3548452 - 318 WP_023087286.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
HV97_RS16690 3548752..3550617 + 1866 WP_023087285.1 TraI domain-containing protein -
HV97_RS16695 3550673..3551095 - 423 WP_063837596.1 MerR family DNA-binding protein -
HV97_RS32675 3551092..3551617 - 526 Protein_3227 hypothetical protein -
HV97_RS16705 3551829..3552230 + 402 WP_023087283.1 hypothetical protein -
HV97_RS16710 3552242..3552481 + 240 WP_023087282.1 DUF2933 domain-containing protein -
HV97_RS16715 3552478..3553128 + 651 WP_023087281.1 isoprenylcysteine carboxylmethyltransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3486532..3601748 115216


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-100)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17660.02 Da        Isoelectric Point: 10.1848

>T47817 WP_009518525.1 NZ_CP008870:c3548135-3547677 [Pseudomonas aeruginosa]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

>T47817 NZ_CP008870:c3548135-3547677 [Pseudomonas aeruginosa]
ATGCAACGGCATGGCTGGACGCTGCTCTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGGCCGCGAGGCG
CGCGCAGGAGAACGATCCGGCGGGCTTCGAGTCCAACGCCAACGTCAAGCTCTTCCGGGCCTTGAGCCAGTTGATGTTGG
ATGTGGTGCCAGGCGATCCGGCACGCGACGAGTACCGCCAGGGCAATACCTTGGGACCTGCCCACCGCCACTGGCGAAGG
GCCAAGATCGGACGGCGGTTCCGGCTGTTCTTCCGGTACGACTCGAAGGCGAAGGTCATTGTGTACGCCTGGGTCAACGA
TGAACAGACCCTGCGGTCTTCGGGTAGCAAATCGGACCCGTATGTCGTGTTCGAGAAGATGCTCGGACGCGGGAACCCAC
CAGACGACTGGCACGCGCTGATACAGGCAAGCAAGCAGGATTGGAGCAAACTGGAATAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11156.69 Da        Isoelectric Point: 4.5685

>AT47817 WP_023087286.1 NZ_CP008870:c3548452-3548135 [Pseudomonas aeruginosa]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNLQGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Download         Length: 318 bp

>AT47817 NZ_CP008870:c3548452-3548135 [Pseudomonas aeruginosa]
ATGCCTGCCATCCACGAAGTCGCCACGTTGACCTCCAAGGGCCAGATCACGCTGCCCAAGCCTATCCGGCAGGCGCTCGG
CGTTGATGCCGGCGGCAAGCTCGCGTTCGATCTGCGGGGCAGCGAAGTCGTCGTCACCCGCGTCGATGCCGAGCACGAGG
ATCCCGCCATCGGCGCGTTCCTGAGCCTGCTGGCCCGAGACATCGAAGCGGGTCGGAACCTCCAGGGCCTGCCCGAGGAT
CTGGCTCGCGCCATGCTGGAGCATGCTGGCCACGGTGTGAACCTGGACGAGGAGATCGACGGGAAAGTGGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ADY6

Antitoxin

Source ID Structure
AlphaFold DB A0A7M2ZSF3

References