Detailed information of TA system TA047811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5704082..5704677
• Replicon: chromosome
• Accession: NZ_CP008869
• Organism: Pseudomonas aeruginosa strain W16407

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HV98_RS27155
• Protein ID: WP_003113526.1
• Coordinates: 5704399..5704677 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV98_RS27150
• Protein ID: WP_003113527.1
• Coordinates: 5704082..5704387 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV98_RS27130	5700158..5700571	+	414	Protein_5267	ATP-binding protein	-
HV98_RS27135	5700791..5701654	-	864	WP_010793758.1	integrase domain-containing protein	-
HV98_RS27140	5702256..5703398	-	1143	WP_010793757.1	hypothetical protein	-
HV98_RS27150	5704082..5704387	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HV98_RS27155	5704399..5704677	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV98_RS27165	5705006..5707234	+	2229	WP_003099265.1	TonB-dependent receptor	-
HV98_RS27170	5707304..5707951	-	648	WP_003095021.1	carbonate dehydratase	-
HV98_RS27175	5708013..5709251	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T47811 WP_003113526.1 NZ_CP008869:c5704677-5704399 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47811 NZ_CP008869:c5704677-5704399 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT47811 WP_003113527.1 NZ_CP008869:c5704387-5704082 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47811 NZ_CP008869:c5704387-5704082 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3