Detailed information of TA system TA047800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 621020..621615
• Replicon: chromosome
• Accession: NZ_CP008868
• Organism: Pseudomonas aeruginosa strain T63266

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HV99_RS29850
• Protein ID: WP_003113526.1
• Coordinates: 621337..621615 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV99_RS02895
• Protein ID: WP_003113527.1
• Coordinates: 621020..621325 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV99_RS02870	616786..617076	-	291	WP_042931660.1	DUF5447 family protein	-
HV99_RS02880	617288..617560	-	273	WP_042931662.1	hypothetical protein	-
HV99_RS02885	617670..617936	+	267	WP_023088595.1	hypothetical protein	-
HV99_RS02890	617992..618327	+	336	WP_042931664.1	hypothetical protein	-
HV99_RS30890	618365..619126	-	762	WP_144440650.1	retron Ec48 family effector membrane protein	-
HV99_RS29845	619071..620279	-	1209	WP_079387228.1	reverse transcriptase family protein	-
HV99_RS02895	621020..621325	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HV99_RS29850	621337..621615	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV99_RS02900	621944..624172	+	2229	WP_003099265.1	TonB-dependent receptor	-
HV99_RS02905	624242..624889	-	648	WP_003095021.1	carbonate dehydratase	-
HV99_RS02910	624951..626189	-	1239	WP_033970863.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T47800 WP_003113526.1 NZ_CP008868:c621615-621337 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47800 NZ_CP008868:c621615-621337 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT47800 WP_003113527.1 NZ_CP008868:c621325-621020 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47800 NZ_CP008868:c621325-621020 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3