Detailed information of TA system TA047795

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 826684..827365
• Replicon: chromosome
• Accession: NZ_CP008867
• Organism: Pseudomonas aeruginosa strain T52373

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: HW00_RS03905
• Protein ID: WP_003111825.1
• Coordinates: 827000..827365 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW00_RS03900
• Protein ID: WP_003145733.1
• Coordinates: 826684..827007 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW00_RS03875	821940..822818	+	879	WP_003109435.1	hypothetical protein	-
HW00_RS28900	823448..825157	+	1710	WP_079758259.1	TIGR04141 family sporadically distributed protein	-
HW00_RS28905	825149..825679	-	531	WP_079758260.1	tyrosine-type recombinase/integrase	-
HW00_RS28910	825787..826062	-	276	WP_004353146.1	hypothetical protein	-
HW00_RS03890	826062..826394	-	333	WP_021205399.1	hypothetical protein	-
HW00_RS29830	826449..826568	+	120	Protein_779	IS5/IS1182 family transposase	-
HW00_RS03900	826684..827007	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
HW00_RS03905	827000..827365	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW00_RS30040	827656..828024	-	369	WP_162471085.1	hypothetical protein	-
HW00_RS03910	828037..828309	+	273	WP_014603216.1	hypothetical protein	-
HW00_RS03915	828339..828764	-	426	WP_003116492.1	VOC family protein	-
HW00_RS03920	828865..829749	+	885	WP_003085663.1	LysR family transcriptional regulator	-
HW00_RS03925	829722..830675	-	954	WP_003085661.1	LysR family transcriptional regulator	-
HW00_RS03930	830896..831285	+	390	WP_059317050.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T47795 WP_003111825.1 NZ_CP008867:c827365-827000 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T47795 NZ_CP008867:c827365-827000 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGATCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT47795 WP_003145733.1 NZ_CP008867:c827007-826684 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT47795 NZ_CP008867:c827007-826684 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAACTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA

Structures

Toxin

• AlphaFold DB: V6AKP0