Detailed information of TA system TA047793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5731968..5732563
• Replicon: chromosome
• Accession: NZ_CP008866
• Organism: Pseudomonas aeruginosa strain T38079

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HW01_RS27275
• Protein ID: WP_003117425.1
• Coordinates: 5732285..5732563 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW01_RS27270
• Protein ID: WP_003099268.1
• Coordinates: 5731968..5732273 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW01_RS27235	5727421..5727711	-	291	WP_023083237.1	DUF5447 family protein	-
HW01_RS27245	5727923..5728195	-	273	WP_003115921.1	hypothetical protein	-
HW01_RS27250	5728305..5728571	+	267	WP_016852153.1	hypothetical protein	-
HW01_RS27255	5728745..5729539	+	795	WP_023082695.1	helix-turn-helix domain-containing protein	-
HW01_RS27260	5729514..5731052	+	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
HW01_RS27265	5731064..5731591	-	528	WP_071535723.1	AAA family ATPase	-
HW01_RS27270	5731968..5732273	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HW01_RS27275	5732285..5732563	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW01_RS27285	5732892..5735120	+	2229	WP_003099265.1	TonB-dependent receptor	-
HW01_RS27290	5735190..5735837	-	648	WP_003095021.1	carbonate dehydratase	-
HW01_RS27295	5735899..5737137	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T47793 WP_003117425.1 NZ_CP008866:c5732563-5732285 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47793 NZ_CP008866:c5732563-5732285 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT47793 WP_003099268.1 NZ_CP008866:c5732273-5731968 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47793 NZ_CP008866:c5732273-5731968 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5