Detailed information of TA system TA047784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5854882..5855477
• Replicon: chromosome
• Accession: NZ_CP008865
• Organism: Pseudomonas aeruginosa strain S86968

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HW02_RS28050
• Protein ID: WP_003117425.1
• Coordinates: 5855199..5855477 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW02_RS28045
• Protein ID: WP_003099268.1
• Coordinates: 5854882..5855187 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW02_RS28020	5850178..5851224	+	1047	WP_145954746.1	GNAT family N-acetyltransferase	-
HW02_RS28025	5851236..5852264	-	1029	WP_034023821.1	IS30 family transposase	-
HW02_RS28030	5852370..5853398	-	1029	WP_034023821.1	IS30 family transposase	-
HW02_RS28035	5853427..5853966	+	540	WP_145954747.1	hypothetical protein	-
HW02_RS28040	5853978..5854505	-	528	WP_071535723.1	AAA family ATPase	-
HW02_RS28045	5854882..5855187	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HW02_RS28050	5855199..5855477	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW02_RS28060	5855806..5858034	+	2229	WP_003099265.1	TonB-dependent receptor	-
HW02_RS28065	5858104..5858751	-	648	WP_003095021.1	carbonate dehydratase	-
HW02_RS28070	5858813..5860051	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T47784 WP_003117425.1 NZ_CP008865:c5855477-5855199 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47784 NZ_CP008865:c5855477-5855199 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT47784 WP_003099268.1 NZ_CP008865:c5855187-5854882 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47784 NZ_CP008865:c5855187-5854882 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5