Detailed information of TA system TA047764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2528515..2529110
• Replicon: chromosome
• Accession: NZ_CP008863
• Organism: Pseudomonas aeruginosa strain M37351

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HW03_RS32495
• Protein ID: WP_003113526.1
• Coordinates: 2528832..2529110 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW03_RS11670
• Protein ID: WP_003133769.1
• Coordinates: 2528515..2528820 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW03_RS33430	2524215..2524796	+	582	WP_123903235.1	sce7726 family protein	-
HW03_RS32485	2524783..2525853	-	1071	WP_023098928.1	beta family protein	-
HW03_RS32490	2525843..2526679	-	837	WP_023098927.1	ImmA/IrrE family metallo-endopeptidase	-
HW03_RS33435	2526672..2527346	-	675	WP_023098926.1	N-acetyltransferase	-
HW03_RS11665	2527772..2528179	-	408	WP_049267485.1	hypothetical protein	-
HW03_RS11670	2528515..2528820	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
HW03_RS32495	2528832..2529110	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW03_RS11675	2529439..2531667	+	2229	WP_003141628.1	TonB-dependent receptor	-
HW03_RS11680	2531737..2532384	-	648	WP_003095021.1	carbonate dehydratase	-
HW03_RS11685	2532446..2533684	-	1239	WP_003141631.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T47764 WP_003113526.1 NZ_CP008863:c2529110-2528832 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47764 NZ_CP008863:c2529110-2528832 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT47764 WP_003133769.1 NZ_CP008863:c2528820-2528515 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47764 NZ_CP008863:c2528820-2528515 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3