Detailed information of TA system TA047753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4911971..4912566
• Replicon: chromosome
• Accession: NZ_CP008861
• Organism: Pseudomonas aeruginosa strain H47921

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HW05_RS32540
• Protein ID: WP_003113526.1
• Coordinates: 4911971..4912249 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW05_RS22520
• Protein ID: WP_003099268.1
• Coordinates: 4912261..4912566 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW05_RS22505	4907397..4908635	+	1239	WP_023108790.1	C69 family dipeptidase	-
HW05_RS22510	4908697..4909344	+	648	WP_003095021.1	carbonate dehydratase	-
HW05_RS22515	4909414..4911642	-	2229	WP_059309786.1	TonB-dependent receptor	-
HW05_RS32540	4911971..4912249	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW05_RS22520	4912261..4912566	+	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HW05_RS22530	4912961..4914061	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HW05_RS22535	4914102..4914686	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HW05_RS22540	4914728..4915342	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HW05_RS22545	4915459..4916400	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HW05_RS22555	4916567..4917415	-	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T47753 WP_003113526.1 NZ_CP008861:4911971-4912249 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47753 NZ_CP008861:4911971-4912249 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTTGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT47753 WP_003099268.1 NZ_CP008861:4912261-4912566 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47753 NZ_CP008861:4912261-4912566 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3