Detailed information of TA system TA047750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 950492..951267
• Replicon: chromosome
• Accession: NZ_CP008861
• Organism: Pseudomonas aeruginosa strain H47921

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V6ADY6
• Locus tag: HW05_RS04125
• Protein ID: WP_009518525.1
• Coordinates: 950492..950950 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V6AEP7
• Locus tag: HW05_RS04130
• Protein ID: WP_023098543.1
• Coordinates: 950950..951267 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW05_RS04100	945833..946780	+	948	WP_023108457.1	TIGR03756 family integrating conjugative element protein	-
HW05_RS04105	946790..948184	+	1395	WP_009518521.1	integrating conjugative element protein	-
HW05_RS04110	948181..948540	+	360	WP_023108458.1	hypothetical protein	-
HW05_RS04115	948555..950072	+	1518	WP_023108459.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
HW05_RS04120	950088..950465	-	378	WP_009518524.1	DUF3742 family protein	-
HW05_RS04125	950492..950950	-	459	WP_009518525.1	type II toxin-antitoxin system YhaV family toxin	Toxin
HW05_RS04130	950950..951267	-	318	WP_023098543.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
HW05_RS04135	951567..953384	+	1818	WP_023108460.1	TraI domain-containing protein	-
HW05_RS31815	953366..953587	-	222	WP_099459023.1	MerR family DNA-binding transcriptional regulator	-
HW05_RS31820	953661..954572	-	912	WP_003090227.1	NAD(P)H-binding protein	-
HW05_RS32945	954646..954843	-	198	WP_023108461.1	hypothetical protein	-
HW05_RS31825	954815..955126	+	312	Protein_840	redox-sensitive transcriptional activator SoxR	-
HW05_RS04150	955361..955783	-	423	WP_003090234.1	MerR family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	940483..987196	46713

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (8-144)

Antitoxin

• PrlF antitoxin (8-100)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17660.02 Da
• Isoelectric Point: 10.1848

>T47750 WP_009518525.1 NZ_CP008861:c950950-950492 [Pseudomonas aeruginosa]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Nucleotide

• Length: 459 bp

>T47750 NZ_CP008861:c950950-950492 [Pseudomonas aeruginosa]
ATGCAACGGCATGGCTGGACGCTGCTCTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGGCCGCGAGGCG
CGCGCAGGAGAATGACCCGGCGGGCTTCGAGTCCAATGCCAACGTCAAGCTCTTCCGGGCCCTGAGCCAGTTGATGTTGG
ATGTGGTGCCAGGCGATCCGGCACGCGACGAGTACCGCCAGGGCAACACCTTGGGACCTGCCCACCGCCACTGGCGAAGG
GCCAAGATCGGACGGCGGTTCCGGCTGTTCTTCCGGTACGACTCGAAGGCGAAGGTCATTGTGTACGCCTGGGTCAACGA
TGAACAGACCCTGCGGTCTTCGGGTAGCAAATCGGACCCGTATGTCGTGTTCGAGAAGATGCTCGGACGCGGGAACCCAC
CAGACGACTGGCACGCGCTGATACAGGCAAGCAAGCAGGATTGGAGCAAACTGGAATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11170.72 Da
• Isoelectric Point: 4.7182

>AT47750 WP_023098543.1 NZ_CP008861:c951267-950950 [Pseudomonas aeruginosa]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNVRGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Nucleotide

• Length: 318 bp

>AT47750 NZ_CP008861:c951267-950950 [Pseudomonas aeruginosa]
ATGCCTGCCATCCACGAAGTCGCCACGCTGACCTCTAAAGGCCAGATCACGCTGCCCAAGCCCATCCGGCAGGCGCTCGG
CGTCGATGCCGGCGGCAAGCTCGCGTTCGATCTGCGGGGCAGCGAAGTCGTCGTCACCCGTGTTGATGCCGAGCACGAGG
ACCCCGCCATTGGCGCGTTTCTGAGCCTGCTGGCCCGCGACATCGAAGCTGGCCGGAACGTCCGGGGCCTGCCCGAGGAT
CTGGCGCGCGCCATGCTGGAGCATGCTGGCCACGGTGTGAACCTGGACGAGGAGATCGACGGGAAAGTGGAACTCTGA

Structures

Toxin

• AlphaFold DB: V6ADY6

Antitoxin

• AlphaFold DB: V6AEP7