Detailed information of TA system TA047748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5541930..5542525
• Replicon: chromosome
• Accession: NZ_CP008860
• Organism: Pseudomonas aeruginosa strain H27930

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HW06_RS26135
• Protein ID: WP_003113526.1
• Coordinates: 5542247..5542525 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW06_RS26130
• Protein ID: WP_003099268.1
• Coordinates: 5541930..5542235 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW06_RS26090	5537111..5537350	+	240	WP_032977980.1	hypothetical protein	-
HW06_RS26095	5537337..5537525	+	189	WP_059309322.1	hypothetical protein	-
HW06_RS31335	5537932..5538090	+	159	WP_186278399.1	hypothetical protein	-
HW06_RS26105	5538093..5538314	-	222	WP_059309324.1	hypothetical protein	-
HW06_RS26110	5538314..5538952	-	639	WP_059309325.1	DNA-binding protein	-
HW06_RS26115	5539995..5541017	-	1023	WP_059309635.1	RepA	-
HW06_RS26120	5541056..5541268	-	213	WP_004359426.1	AlpA family phage regulatory protein	-
HW06_RS26130	5541930..5542235	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HW06_RS26135	5542247..5542525	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW06_RS26145	5542854..5545082	+	2229	WP_003099265.1	TonB-dependent receptor	-
HW06_RS26150	5545152..5545799	-	648	WP_003095021.1	carbonate dehydratase	-
HW06_RS26155	5545861..5547099	-	1239	WP_019681675.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T47748 WP_003113526.1 NZ_CP008860:c5542525-5542247 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47748 NZ_CP008860:c5542525-5542247 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT47748 WP_003099268.1 NZ_CP008860:c5542235-5541930 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47748 NZ_CP008860:c5542235-5541930 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3