Detailed information of TA system TA047742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5300993..5301588
• Replicon: chromosome
• Accession: NZ_CP008859
• Organism: Pseudomonas aeruginosa strain H5708

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HW07_RS25060
• Protein ID: WP_003113526.1
• Coordinates: 5301310..5301588 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW07_RS25055
• Protein ID: WP_003113527.1
• Coordinates: 5300993..5301298 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW07_RS25035	5296391..5296726	+	336	WP_025921265.1	hypothetical protein	-
HW07_RS25040	5296736..5296978	+	243	WP_080712179.1	NUDIX domain-containing protein	-
HW07_RS25045	5297052..5299280	-	2229	WP_080712178.1	ATP-binding protein	-
HW07_RS25050	5299284..5300369	-	1086	WP_031631918.1	DNA cytosine methyltransferase	-
HW07_RS25055	5300993..5301298	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HW07_RS25060	5301310..5301588	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW07_RS25070	5301917..5304145	+	2229	WP_003099265.1	TonB-dependent receptor	-
HW07_RS25075	5304216..5304863	-	648	WP_003095021.1	carbonate dehydratase	-
HW07_RS25080	5304925..5306163	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T47742 WP_003113526.1 NZ_CP008859:c5301588-5301310 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47742 NZ_CP008859:c5301588-5301310 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT47742 WP_003113527.1 NZ_CP008859:c5301298-5300993 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47742 NZ_CP008859:c5301298-5300993 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3