Detailed information of TA system TA047737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5573719..5574314
• Replicon: chromosome
• Accession: NZ_CP008858
• Organism: Pseudomonas aeruginosa strain F63912

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HW08_RS26900
• Protein ID: WP_003117425.1
• Coordinates: 5574036..5574314 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW08_RS26895
• Protein ID: WP_003113527.1
• Coordinates: 5573719..5574024 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW08_RS26860	5568858..5569706	+	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HW08_RS26870	5569873..5570814	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HW08_RS26875	5570931..5571545	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HW08_RS26880	5571587..5572171	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HW08_RS26885	5572212..5573312	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HW08_RS26895	5573719..5574024	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HW08_RS26900	5574036..5574314	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW08_RS26910	5574643..5576871	+	2229	WP_003113525.1	TonB-dependent receptor	-
HW08_RS26915	5576941..5577588	-	648	WP_003095021.1	carbonate dehydratase	-
HW08_RS26920	5577650..5578888	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T47737 WP_003117425.1 NZ_CP008858:c5574314-5574036 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47737 NZ_CP008858:c5574314-5574036 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT47737 WP_003113527.1 NZ_CP008858:c5574024-5573719 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47737 NZ_CP008858:c5574024-5573719 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5