Detailed information of TA system TA047735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4857393..4858074
• Replicon: chromosome
• Accession: NZ_CP008858
• Organism: Pseudomonas aeruginosa strain F63912

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HW08_RS23485
• Protein ID: WP_015503432.1
• Coordinates: 4857709..4858074 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW08_RS23480
• Protein ID: WP_016561576.1
• Coordinates: 4857393..4857716 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW08_RS23445	4853285..4853968	-	684	WP_003085795.1	TetR/AcrR family transcriptional regulator	-
HW08_RS23450	4854056..4854934	+	879	WP_003109435.1	hypothetical protein	-
HW08_RS23460	4855537..4855764	+	228	WP_014603215.1	hypothetical protein	-
HW08_RS23465	4855779..4856321	-	543	WP_019396018.1	tyrosine-type recombinase/integrase	-
HW08_RS23470	4856496..4856771	-	276	WP_004353146.1	hypothetical protein	-
HW08_RS23475	4856771..4857103	-	333	WP_016561578.1	hypothetical protein	-
HW08_RS31960	4857158..4857277	+	120	Protein_4539	IS5/IS1182 family transposase	-
HW08_RS23480	4857393..4857716	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
HW08_RS23485	4857709..4858074	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW08_RS23490	4858338..4858580	-	243	WP_043884955.1	hypothetical protein	-
HW08_RS23495	4858787..4859059	+	273	WP_003085667.1	hypothetical protein	-
HW08_RS23500	4859078..4859503	-	426	WP_003114206.1	ring-cleaving dioxygenase	-
HW08_RS23505	4859604..4860488	+	885	WP_003454589.1	LysR family transcriptional regulator	-
HW08_RS23510	4860461..4861414	-	954	WP_003085661.1	LysR family transcriptional regulator	-
HW08_RS23515	4861635..4862069	+	435	WP_016253806.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T47735 WP_015503432.1 NZ_CP008858:c4858074-4857709 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T47735 NZ_CP008858:c4858074-4857709 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT47735 WP_016561576.1 NZ_CP008858:c4857716-4857393 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT47735 NZ_CP008858:c4857716-4857393 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA