Detailed information of TA system TA047727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3509691..3510286
• Replicon: chromosome
• Accession: NZ_CP008857
• Organism: Pseudomonas aeruginosa strain F30658

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HW09_RS34335
• Protein ID: WP_003117425.1
• Coordinates: 3510008..3510286 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW09_RS16140
• Protein ID: WP_003099268.1
• Coordinates: 3509691..3509996 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW09_RS16105	3504830..3505678	+	849	WP_003162406.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HW09_RS16115	3505845..3506786	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HW09_RS16120	3506903..3507517	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HW09_RS16125	3507559..3508143	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HW09_RS16130	3508184..3509284	+	1101	WP_049244135.1	redox-regulated ATPase YchF	-
HW09_RS16140	3509691..3509996	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HW09_RS34335	3510008..3510286	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW09_RS16145	3510611..3512839	+	2229	WP_003113525.1	TonB-dependent receptor	-
HW09_RS16150	3512909..3513556	-	648	WP_003095021.1	carbonate dehydratase	-
HW09_RS16155	3513618..3514856	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T47727 WP_003117425.1 NZ_CP008857:c3510286-3510008 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47727 NZ_CP008857:c3510286-3510008 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT47727 WP_003099268.1 NZ_CP008857:c3509996-3509691 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47727 NZ_CP008857:c3509996-3509691 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5