Detailed information of TA system TA047718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4764867..4765548
• Replicon: chromosome
• Accession: NZ_CP008856
• Organism: Pseudomonas aeruginosa strain F23197

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: HW10_RS22400
• Protein ID: WP_003111825.1
• Coordinates: 4765183..4765548 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW10_RS22395
• Protein ID: WP_003145733.1
• Coordinates: 4764867..4765190 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW10_RS22370	4760780..4761418	+	639	WP_003140882.1	hypothetical protein	-
HW10_RS22375	4761661..4761996	+	336	WP_003140884.1	TM2 domain-containing protein	-
HW10_RS22380	4762170..4762688	+	519	WP_034012953.1	PAAR domain-containing protein	-
HW10_RS22385	4762685..4763386	+	702	WP_030046795.1	VRR-NUC domain-containing protein	-
HW10_RS22390	4763403..4764491	+	1089	WP_033936233.1	DUF3396 domain-containing protein	-
HW10_RS22395	4764867..4765190	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
HW10_RS22400	4765183..4765548	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW10_RS22405	4765812..4766051	-	240	WP_044060930.1	hypothetical protein	-
HW10_RS22410	4766258..4766530	+	273	WP_003085667.1	hypothetical protein	-
HW10_RS22415	4766549..4766974	-	426	WP_003085665.1	ring-cleaving dioxygenase	-
HW10_RS22420	4767075..4767959	+	885	WP_023084912.1	LysR family transcriptional regulator	-
HW10_RS22425	4767932..4768885	-	954	WP_003085661.1	LysR family transcriptional regulator	-
HW10_RS22430	4769106..4769540	+	435	WP_003116494.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4761661..4767959	6298

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T47718 WP_003111825.1 NZ_CP008856:c4765548-4765183 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T47718 NZ_CP008856:c4765548-4765183 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGAGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT47718 WP_003145733.1 NZ_CP008856:c4765190-4764867 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT47718 NZ_CP008856:c4765190-4764867 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATCGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA

Structures

Toxin

• AlphaFold DB: V6AKP0