Detailed information of TA system TA047703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 150309..151036
• Replicon: plasmid pKOXM1A
• Accession: NZ_CP008842
• Organism: Klebsiella michiganensis strain M1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LNL2
• Locus tag: HR38_RS27835
• Protein ID: WP_004118600.1
• Coordinates: 150309..150620 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: HR38_RS27840
• Protein ID: WP_000990392.1
• Coordinates: 150617..151036 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR38_RS27815	146823..147542	+	720	WP_001102107.1	(Fe-S)-binding protein	-
HR38_RS27820	147553..148980	+	1428	WP_000023895.1	iron-sulfur cluster-binding protein	-
HR38_RS27825	148973..149668	+	696	WP_004118595.1	lactate utilization protein C	-
HR38_RS27830	149718..150098	-	381	Protein_157	gluconate permease	-
HR38_RS27835	150309..150620	+	312	WP_004118600.1	type II toxin-antitoxin system HigB family toxin	Toxin
HR38_RS27840	150617..151036	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
HR38_RS27845	151073..152275	-	1203	WP_003031541.1	hypothetical protein	-
HR38_RS27850	152268..152597	-	330	WP_004118613.1	hypothetical protein	-
HR38_RS27855	152594..153253	-	660	WP_000078540.1	hypothetical protein	-
HR38_RS32120	153677..154270	-	594	WP_044348674.1	hypothetical protein	-
HR38_RS27865	154586..154939	+	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
HR38_RS27870	154987..155349	+	363	WP_000783215.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..204468	204468

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12386.14 Da
• Isoelectric Point: 10.0935

>T47703 WP_004118600.1 NZ_CP008842:150309-150620 [Klebsiella michiganensis]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T47703 NZ_CP008842:150309-150620 [Klebsiella michiganensis]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT47703 WP_000990392.1 NZ_CP008842:150617-151036 [Klebsiella michiganensis]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT47703 NZ_CP008842:150617-151036 [Klebsiella michiganensis]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGATCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LNL2

Antitoxin

• AlphaFold DB: V0AHC4