47690

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1005905..1006555 Replicon chromosome
Accession NZ_CP008841
Organism Klebsiella michiganensis strain M1

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag HR38_RS04765 Protein ID WP_014227314.1
Coordinates 1006214..1006555 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag HR38_RS04760 Protein ID WP_014227313.1
Coordinates 1005905..1006204 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR38_RS04740 1001790..1002509 - 720 WP_032718745.1 phosphonate utilization transcriptional regulator PhnR -
HR38_RS04745 1002650..1003753 + 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
HR38_RS04750 1003763..1004572 + 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
HR38_RS04755 1004605..1005786 - 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
HR38_RS04760 1005905..1006204 - 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
HR38_RS04765 1006214..1006555 - 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HR38_RS04770 1006806..1007258 - 453 WP_014839842.1 DUF1198 domain-containing protein -
HR38_RS04775 1007707..1009098 - 1392 WP_004126843.1 hexose-6-phosphate:phosphate antiporter -
HR38_RS04780 1009244..1010563 - 1320 WP_014227316.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T47690 WP_014227314.1 NZ_CP008841:c1006555-1006214 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T47690 NZ_CP008841:c1006555-1006214 [Klebsiella michiganensis]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT47690 WP_014227313.1 NZ_CP008841:c1006204-1005905 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT47690 NZ_CP008841:c1006204-1005905 [Klebsiella michiganensis]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AGCCCACCATTGCTAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References