Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 5032327..5032913 Replicon chromosome
Accession NZ_CP008827
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH1

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KPNIH1_RS25530 Protein ID WP_002920800.1
Coordinates 5032327..5032695 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag KPNIH1_RS25535 Protein ID WP_002920802.1
Coordinates 5032692..5032913 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH1_RS25510 5027830..5028900 - 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
KPNIH1_RS25515 5028902..5029747 - 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KPNIH1_RS25520 5029744..5030631 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KPNIH1_RS25525 5030738..5032054 - 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KPNIH1_RS25530 5032327..5032695 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KPNIH1_RS25535 5032692..5032913 - 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KPNIH1_RS25540 5033077..5033790 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KPNIH1_RS25545 5033792..5034559 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KPNIH1_RS25550 5034556..5035833 - 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
KPNIH1_RS25555 5035830..5036756 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 5027093..5047681 20588


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T47662 WP_002920800.1 NZ_CP008827:c5032695-5032327 [Klebsiella pneumoniae subsp. pneumoniae KPNIH1]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T47662 NZ_CP008827:c5032695-5032327 [Klebsiella pneumoniae subsp. pneumoniae KPNIH1]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT47662 WP_002920802.1 NZ_CP008827:c5032913-5032692 [Klebsiella pneumoniae subsp. pneumoniae KPNIH1]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT47662 NZ_CP008827:c5032913-5032692 [Klebsiella pneumoniae subsp. pneumoniae KPNIH1]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References