Detailed information of TA system TA047638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3667629..3668304
• Replicon: chromosome
• Accession: NZ_CP008823
• Organism: UNVERIFIED_ORG: Enterobacter cloacae ECNIH2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ECNIH2_RS17555
• Protein ID: WP_032619177.1
• Coordinates: 3667629..3667928 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: ECNIH2_RS17560
• Protein ID: WP_015571639.1
• Coordinates: 3667939..3668304 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECNIH2_RS17540 (ECNIH2_17655)	3663542..3664510	-	969	WP_080288382.1	IS5-like element IS903B family transposase	-
ECNIH2_RS17545 (ECNIH2_17660)	3664543..3666198	+	1656	Protein_3461	ATP-binding cassette domain-containing protein	-
ECNIH2_RS17550 (ECNIH2_17665)	3666179..3667378	+	1200	WP_017382757.1	HlyD family efflux transporter periplasmic adaptor subunit	-
ECNIH2_RS17555 (ECNIH2_17670)	3667629..3667928	+	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECNIH2_RS17560 (ECNIH2_17675)	3667939..3668304	+	366	WP_015571639.1	HigA family addiction module antitoxin	Antitoxin
ECNIH2_RS17565 (ECNIH2_17680)	3668331..3669512	-	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
ECNIH2_RS17570 (ECNIH2_17685)	3669533..3670420	-	888	WP_032619173.1	LysR family transcriptional regulator	-
ECNIH2_RS17575 (ECNIH2_17690)	3670518..3671120	+	603	WP_017382759.1	short chain dehydrogenase	-
ECNIH2_RS17580 (ECNIH2_17695)	3671117..3671848	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3631173..3668864	37691
	-	flank	IS/Tn	-	-	3663542..3664510	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T47638 WP_032619177.1 NZ_CP008823:3667629-3667928 [Enterobacter cloacae ECNIH2]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T47638 NZ_CP008823:3667629-3667928 [Enterobacter cloacae ECNIH2]
ATGATTAATCATTCTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT47638 WP_015571639.1 NZ_CP008823:3667939-3668304 [Enterobacter cloacae ECNIH2]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT47638 NZ_CP008823:3667939-3668304 [Enterobacter cloacae ECNIH2]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9