Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3052498..3053158 Replicon chromosome
Accession NZ_CP008823
Organism UNVERIFIED_ORG: Enterobacter cloacae ECNIH2

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q4X0
Locus tag ECNIH2_RS14775 Protein ID WP_017383312.1
Coordinates 3052805..3053158 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q4Z7
Locus tag ECNIH2_RS14770 Protein ID WP_017383313.1
Coordinates 3052498..3052800 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH2_RS14755 (ECNIH2_14835) 3048672..3049997 + 1326 WP_032619371.1 SidA/IucD/PvdA family monooxygenase -
ECNIH2_RS14760 (ECNIH2_14840) 3050024..3052213 + 2190 WP_023303946.1 TonB-dependent siderophore receptor -
ECNIH2_RS14770 (ECNIH2_14850) 3052498..3052800 - 303 WP_017383313.1 XRE family transcriptional regulator Antitoxin
ECNIH2_RS14775 (ECNIH2_14855) 3052805..3053158 - 354 WP_017383312.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECNIH2_RS14780 (ECNIH2_14860) 3053349..3054299 - 951 WP_032619370.1 HTH-type transcriptional regulator Cbl -
ECNIH2_RS14785 (ECNIH2_14865) 3054396..3055313 - 918 WP_003859531.1 nitrogen assimilation transcriptional regulator NAC -
ECNIH2_RS14795 (ECNIH2_14875) 3055845..3056777 - 933 WP_023303950.1 L,D-transpeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(18-89)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13537.37 Da        Isoelectric Point: 9.9538

>T47637 WP_017383312.1 NZ_CP008823:c3053158-3052805 [Enterobacter cloacae ECNIH2]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLRERGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREYTRWLNTLKERN

Download         Length: 354 bp

>T47637 NZ_CP008823:c3053158-3052805 [Enterobacter cloacae ECNIH2]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTTTTGCGGGAGAGGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAGTTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCGATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGGTCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11028.85 Da        Isoelectric Point: 6.4727

>AT47637 WP_017383313.1 NZ_CP008823:c3052800-3052498 [Enterobacter cloacae ECNIH2]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT47637 NZ_CP008823:c3052800-3052498 [Enterobacter cloacae ECNIH2]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGACGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTACCCGATGGTTCTCATTACGAGTTTGTGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q4X0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q4Z7

References