Detailed information of TA system TA047621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 34419..35009
• Replicon: plasmid 2
• Accession: NZ_CP008818
• Organism: Brucella anthropi strain OAB

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A6X893
• Locus tag: DR92_RS01010
• Protein ID: WP_011983054.1
• Coordinates: 34728..35009 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DR92_RS01005
• Protein ID: WP_011983055.1
• Coordinates: 34419..34715 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR92_RS00980	30075..31090	+	1016	Protein_30	IS3 family transposase	-
DR92_RS00985	31106..31423	-	318	WP_040128186.1	hypothetical protein	-
DR92_RS00990	31518..32156	-	639	WP_011983059.1	hypothetical protein	-
DR92_RS01000	33528..34040	-	513	WP_011983057.1	hypothetical protein	-
DR92_RS01005	34419..34715	-	297	WP_011983055.1	HigA family addiction module antidote protein	Antitoxin
DR92_RS01010	34728..35009	-	282	WP_011983054.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DR92_RS23315	35171..35800	+	630	WP_011983053.1	SH3 domain-containing protein	-
DR92_RS01015	36054..36434	-	381	WP_011983052.1	DUF2195 family protein	-
DR92_RS01020	36449..36874	-	426	WP_011983051.1	DUF5086 domain-containing protein	-
DR92_RS01025	36976..38199	-	1224	WP_011983050.1	replication initiation protein RepC	-
DR92_RS01030	38354..39397	-	1044	WP_011983049.1	plasmid partitioning protein RepB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106739	106739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10479.00 Da
• Isoelectric Point: 9.0323

>T47621 WP_011983054.1 NZ_CP008818:c35009-34728 [Brucella anthropi]
MIRSYKDARTEAVAAGKAPKGFPADLIRTTVRKLTMIDSAVILDDLRSPPGNRLEALKGDRKGQHSIRINDQWRICFVWT
DAGAEQIEIVDYH

Nucleotide

• Length: 282 bp

>T47621 NZ_CP008818:c35009-34728 [Brucella anthropi]
ATGATCCGTTCTTACAAAGATGCCAGAACCGAAGCCGTTGCAGCCGGCAAAGCGCCAAAGGGGTTCCCGGCAGATTTGAT
AAGAACAACTGTTCGCAAACTTACCATGATCGATAGTGCTGTAATTCTCGATGACCTTCGCTCTCCTCCCGGAAACAGGC
TGGAAGCTCTGAAGGGGGACCGTAAAGGGCAGCACTCGATTCGCATCAACGATCAGTGGCGCATCTGTTTCGTTTGGACC
GATGCTGGCGCTGAACAAATCGAAATTGTTGACTATCACTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10929.70 Da
• Isoelectric Point: 7.2026

>AT47621 WP_011983055.1 NZ_CP008818:c34715-34419 [Brucella anthropi]
MTSKLPPVHPGEILREEYLIPLGMSAGALAKNLNVPRTRIERLATEQTAVTPDTALRLAKFFRTTPEFWMNLQSGYDLKT
EAVAIQDQLLAIHELKAA

Nucleotide

• Length: 297 bp

>AT47621 NZ_CP008818:c34715-34419 [Brucella anthropi]
ATGACCAGTAAACTCCCCCCGGTCCATCCCGGCGAAATCCTTCGGGAAGAATATCTCATCCCGCTCGGGATGAGTGCAGG
TGCGCTTGCCAAGAATCTGAATGTTCCTCGCACTCGAATTGAGCGTCTGGCGACAGAACAGACTGCCGTTACACCTGATA
CGGCTCTGCGCCTTGCGAAGTTCTTCCGCACCACGCCCGAATTCTGGATGAATTTGCAGTCAGGTTACGACCTTAAAACA
GAGGCGGTAGCTATTCAGGATCAGCTTTTAGCCATCCATGAACTGAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A6X893