Detailed information of TA system TA047565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4071126..4071925
• Replicon: chromosome
• Accession: NZ_CP008805
• Organism: Escherichia coli O157:H7 str. SS17

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: SS17_RS21265
• Protein ID: WP_038431117.1
• Coordinates: 4071461..4071925 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: SS17_RS21260
• Protein ID: WP_001302819.1
• Coordinates: 4071126..4071461 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SS17_RS21240	4066911..4067681	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
SS17_RS21245	4067697..4069031	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
SS17_RS21255	4069406..4070977	+	1572	WP_001273776.1	galactarate dehydratase	-
SS17_RS21260	4071126..4071461	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
SS17_RS21265	4071461..4071925	+	465	WP_038431117.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
SS17_RS21270	4071980..4072789	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
SS17_RS21275	4073038..4074318	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
SS17_RS21280	4074341..4074814	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
SS17_RS21285	4074825..4075604	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
SS17_RS21290	4075594..4076472	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
SS17_RS21295	4076490..4076924	+	435	WP_000948831.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17863.28 Da
• Isoelectric Point: 9.6924

>T47565 WP_038431117.1 NZ_CP008805:4071461-4071925 [Escherichia coli O157:H7 str. SS17]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKQDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T47565 NZ_CP008805:4071461-4071925 [Escherichia coli O157:H7 str. SS17]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAACAAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT47565 WP_001302819.1 NZ_CP008805:4071126-4071461 [Escherichia coli O157:H7 str. SS17]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT47565 NZ_CP008805:4071126-4071461 [Escherichia coli O157:H7 str. SS17]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA