Detailed information of TA system TA047548

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1746888..1747113
• Replicon: chromosome
• Accession: NZ_CP008805
• Organism: Escherichia coli O157:H7 str. SS17

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: SS17_RS08965
• Protein ID: WP_000813254.1
• Coordinates: 1746958..1747113 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1746888..1746946 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SS17_RS33235	1742342..1743382	+	1041	WP_000020570.1	DNA-binding protein	-
SS17_RS33240	1743294..1743836	+	543	WP_157837342.1	replication protein	-
SS17_RS08935	1743871..1744641	+	771	WP_000537576.1	DUF1627 domain-containing protein	-
SS17_RS08940	1744657..1745052	+	396	WP_001118161.1	DUF977 family protein	-
SS17_RS08945	1745109..1745465	+	357	WP_001302146.1	hypothetical protein	-
SS17_RS08950	1745514..1745726	+	213	WP_000063625.1	hypothetical protein	-
SS17_RS08955	1745762..1746133	+	372	WP_000137941.1	hypothetical protein	-
SS17_RS08960	1746130..1746492	+	363	WP_000610377.1	DUF551 domain-containing protein	-
SS17_RS32195	1746608..1746712	+	105	WP_001278450.1	hypothetical protein	-
-	1746888..1746946	-	59	-	-	Antitoxin
SS17_RS08965	1746958..1747113	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
SS17_RS08970	1747333..1747590	+	258	WP_001217394.1	hypothetical protein	-
SS17_RS32200	1747660..1747938	+	279	WP_012779355.1	hypothetical protein	-
SS17_RS08980	1747940..1748989	+	1050	WP_038430946.1	DUF968 domain-containing protein	-
SS17_RS08985	1749002..1749376	+	375	WP_038430948.1	RusA family crossover junction endodeoxyribonuclease	-
SS17_RS08990	1749373..1750194	+	822	WP_000762860.1	antitermination protein	-
SS17_RS33245	1750421..1750618	+	198	WP_000917735.1	hypothetical protein	-
SS17_RS09000	1750769..1751827	+	1059	WP_000483509.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / nleH2 / nleF / espM1 / ospG	1673099..1782250	109151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T47548 WP_000813254.1 NZ_CP008805:1746958-1747113 [Escherichia coli O157:H7 str. SS17]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T47548 NZ_CP008805:1746958-1747113 [Escherichia coli O157:H7 str. SS17]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT47548 NZ_CP008805:c1746946-1746888 [Escherichia coli O157:H7 str. SS17]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin