Detailed information of TA system TA047518

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-ralA/-
• Location: 3019270..3019691
• Replicon: chromosome
• Accession: NZ_CP008801
• Organism: Escherichia coli KLY

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: HQ24_RS14970
• Protein ID: WP_001302184.1
• Coordinates: 3019533..3019691 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3019270..3019468 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ24_RS14940	3015200..3015739	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
HQ24_RS14945	3015796..3016029	+	234	WP_000005990.1	DUF905 family protein	-
HQ24_RS14950	3016095..3018053	+	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
HQ24_RS14955	3018108..3018542	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
HQ24_RS14960	3018539..3019258	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	3019270..3019468	+	199	NuclAT_1	-	Antitoxin
-	3019270..3019468	+	199	NuclAT_1	-	Antitoxin
-	3019270..3019468	+	199	NuclAT_1	-	Antitoxin
-	3019270..3019468	+	199	NuclAT_1	-	Antitoxin
HQ24_RS14970	3019533..3019691	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
HQ24_RS26645	3019929..3020288	-	360	Protein_2849	hypothetical protein	-
HQ24_RS26650	3020358..3020564	+	207	WP_000547965.1	hypothetical protein	-
HQ24_RS14985	3020589..3020876	+	288	WP_000107542.1	hypothetical protein	-
HQ24_RS14990	3020995..3021816	+	822	WP_001234417.1	DUF945 domain-containing protein	-
HQ24_RS14995	3022112..3022702	-	591	WP_170919186.1	transglycosylase SLT domain-containing protein	-
HQ24_RS15000	3023035..3023418	+	384	WP_001151527.1	relaxosome protein TraM	-
HQ24_RS15005	3023605..3024294	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2974230..3074293	100063

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T47518 WP_001302184.1 NZ_CP008801:3019533-3019691 [Escherichia coli KLY]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T47518 NZ_CP008801:3019533-3019691 [Escherichia coli KLY]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT47518 NZ_CP008801:3019270-3019468 [Escherichia coli KLY]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin