Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2064916..2065418 Replicon chromosome
Accession NZ_CP008801
Organism Escherichia coli KLY

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HQ24_RS10395 Protein ID WP_000767829.1
Coordinates 2064916..2065170 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HQ24_RS10400 Protein ID WP_001259255.1
Coordinates 2065167..2065418 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQ24_RS10370 2059931..2060158 - 228 WP_000234896.1 sulfurtransferase TusA -
HQ24_RS10375 2060172..2061230 - 1059 WP_000492339.1 transport protein YeeE -
HQ24_RS10380 2061409..2062767 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HQ24_RS26235 2062757..2062819 - 63 WP_010723108.1 membrane protein YoeI -
HQ24_RS10385 2063034..2063963 - 930 WP_000803351.1 LysR family transcriptional regulator -
HQ24_RS10390 2064009..2064833 - 825 WP_000754737.1 SDR family oxidoreductase -
HQ24_RS10395 2064916..2065170 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HQ24_RS10400 2065167..2065418 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HQ24_RS24470 2065701..2065751 + 51 WP_001364200.1 his operon leader peptide -
HQ24_RS10405 2065897..2066796 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HQ24_RS10410 2066802..2068106 + 1305 WP_000009594.1 histidinol dehydrogenase -
HQ24_RS10415 2068103..2069173 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
HQ24_RS10420 2069173..2070240 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T47513 WP_000767829.1 NZ_CP008801:c2065170-2064916 [Escherichia coli KLY]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T47513 NZ_CP008801:c2065170-2064916 [Escherichia coli KLY]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT47513 WP_001259255.1 NZ_CP008801:c2065418-2065167 [Escherichia coli KLY]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT47513 NZ_CP008801:c2065418-2065167 [Escherichia coli KLY]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References