Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 6047234..6047884 Replicon chromosome
Accession NZ_CP008788
Organism Klebsiella oxytoca KONIH1

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag KONIH1_RS28320 Protein ID WP_014227314.1
Coordinates 6047543..6047884 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag KONIH1_RS28315 Protein ID WP_014227313.1
Coordinates 6047234..6047533 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KONIH1_RS28295 6043119..6043838 - 720 WP_042946139.1 phosphonate utilization transcriptional regulator PhnR -
KONIH1_RS28300 6043979..6045082 + 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
KONIH1_RS28305 6045092..6045901 + 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
KONIH1_RS28310 6045934..6047115 - 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
KONIH1_RS28315 6047234..6047533 - 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
KONIH1_RS28320 6047543..6047884 - 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KONIH1_RS28325 6048135..6048587 - 453 WP_032718748.1 DUF1198 domain-containing protein -
KONIH1_RS28330 6049036..6050427 - 1392 WP_004107098.1 hexose-6-phosphate:phosphate antiporter -
KONIH1_RS28335 6050573..6051892 - 1320 WP_032751824.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T47460 WP_014227314.1 NZ_CP008788:c6047884-6047543 [Klebsiella oxytoca KONIH1]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T47460 NZ_CP008788:c6047884-6047543 [Klebsiella oxytoca KONIH1]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT47460 WP_014227313.1 NZ_CP008788:c6047533-6047234 [Klebsiella oxytoca KONIH1]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT47460 NZ_CP008788:c6047533-6047234 [Klebsiella oxytoca KONIH1]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AACCCACCATAGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References