Detailed information of TA system TA047439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1962868..1963525
• Replicon: chromosome
• Accession: NZ_CP008785
• Organism: Burkholderia thailandensis E264

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0F6L6G8
• Locus tag: DR63_RS09670
• Protein ID: WP_009910242.1
• Coordinates: 1962868..1963200 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DR63_RS09675
• Protein ID: WP_011402654.1
• Coordinates: 1963208..1963525 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR63_RS09645	1959257..1959838	+	582	WP_043300356.1	hypothetical protein	-
DR63_RS09650	1960297..1961076	+	780	WP_011402651.1	hypothetical protein	-
DR63_RS09665	1962284..1962826	+	543	WP_102860125.1	site-specific integrase	-
DR63_RS09670	1962868..1963200	+	333	WP_009910242.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DR63_RS09675	1963208..1963525	+	318	WP_011402654.1	helix-turn-helix domain-containing protein	Antitoxin
DR63_RS09685	1963777..1964265	+	489	Protein_1713	site-specific integrase	-
DR63_RS09690	1964379..1965344	-	966	Protein_1714	IS256 family transposase	-
DR63_RS09695	1965424..1965984	+	561	Protein_1715	LysR family transcriptional regulator	-
DR63_RS09700	1965950..1966498	+	549	WP_080511571.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
DR63_RS09705	1966431..1967222	-	792	WP_009910252.1	ethanolamine ammonia-lyase subunit EutC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1961477..1965368	3891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-97)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12849.92 Da
• Isoelectric Point: 10.3619

>T47439 WP_009910242.1 NZ_CP008785:1962868-1963200 [Burkholderia thailandensis E264]
MKFDWTVVYYNERVKRDVFAFPAGILADYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPKGREGIGRVFYCTHVGRQVV
VLHSFVKKTQETPQHELRIAQARLREVRNG

Nucleotide

• Length: 333 bp

>T47439 NZ_CP008785:1962868-1963200 [Burkholderia thailandensis E264]
ATGAAATTTGATTGGACGGTCGTCTACTACAACGAACGGGTGAAGCGCGACGTTTTCGCGTTCCCGGCTGGGATTCTCGC
GGACTACCTCCGGCTGTTGGATCTGATGCAGGAATTCGGGGCGGATCTACGGATGCCGCATTCCAGAGCAATGGGTAACG
GGCTGTTTGAGTTGCGCCCCAAGGGTAGGGAAGGCATCGGGCGGGTGTTCTATTGCACGCATGTGGGGCGGCAAGTCGTT
GTGCTGCACTCCTTCGTCAAGAAGACACAGGAAACGCCGCAACATGAATTGCGGATCGCCCAAGCACGTTTGAGAGAGGT
GCGTAATGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11277.80 Da
• Isoelectric Point: 10.2764

>AT47439 WP_011402654.1 NZ_CP008785:1963208-1963525 [Burkholderia thailandensis E264]
VKRVRAEGFNPVPHTADDTARLLAGRNVKAAYDALEDEYTALRAILSARRDAGLTQAQIAERMGTTASAVSRLEASLSSE
KHSPSFATLRKYAAACGKRLVISFA

Nucleotide

• Length: 318 bp

>AT47439 NZ_CP008785:1963208-1963525 [Burkholderia thailandensis E264]
GTGAAGCGTGTTCGCGCTGAGGGATTCAATCCGGTTCCGCATACGGCGGACGACACGGCGCGCCTGCTCGCCGGTCGCAA
TGTCAAGGCGGCGTATGACGCGTTGGAGGATGAGTACACCGCGTTGCGCGCTATCTTGTCTGCACGGCGCGATGCTGGTC
TGACACAGGCTCAGATTGCTGAGCGGATGGGTACGACCGCATCGGCGGTCTCGCGGCTTGAGGCTTCGTTGTCGAGTGAA
AAGCATTCACCATCATTTGCGACGCTGCGTAAATACGCGGCTGCATGTGGTAAGCGGCTTGTGATTTCGTTTGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0F6L6G8