Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3484871..3485541 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag GS11_RS16400 Protein ID WP_003899954.1
Coordinates 3484871..3485215 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag GS11_RS16405 Protein ID WP_003899955.1
Coordinates 3485212..3485541 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS16370 3479944..3480804 + 861 WP_003416628.1 alpha/beta hydrolase -
GS11_RS16375 3480779..3481294 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
GS11_RS16380 3481534..3481827 + 294 WP_003416635.1 hypothetical protein -
GS11_RS16385 3482115..3483404 + 1290 WP_003416640.1 ATP-binding protein -
GS11_RS16390 3483751..3484185 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
GS11_RS16395 3484188..3484640 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GS11_RS16400 3484871..3485215 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GS11_RS16405 3485212..3485541 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
GS11_RS16410 3486079..3486426 + 348 WP_003899956.1 DUF2384 domain-containing protein -
GS11_RS16415 3486423..3487043 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
GS11_RS16420 3487203..3488468 - 1266 WP_003909837.1 hypothetical protein -
GS11_RS16425 3488636..3488845 + 210 WP_003416778.1 hypothetical protein -
GS11_RS16435 3489092..3490126 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T47397 WP_003899954.1 NZ_CP008744:3484871-3485215 [Mycobacterium tuberculosis variant bovis BCG]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T47397 NZ_CP008744:3484871-3485215 [Mycobacterium tuberculosis variant bovis BCG]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT47397 WP_003899955.1 NZ_CP008744:3485212-3485541 [Mycobacterium tuberculosis variant bovis BCG]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT47397 NZ_CP008744:3485212-3485541 [Mycobacterium tuberculosis variant bovis BCG]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References