Detailed information of TA system TA047397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3484871..3485541
• Replicon: chromosome
• Accession: NZ_CP008744
• Organism: Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: GS11_RS16400
• Protein ID: WP_003899954.1
• Coordinates: 3484871..3485215 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: GS11_RS16405
• Protein ID: WP_003899955.1
• Coordinates: 3485212..3485541 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS11_RS16370	3479944..3480804	+	861	WP_003416628.1	alpha/beta hydrolase	-
GS11_RS16375	3480779..3481294	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
GS11_RS16380	3481534..3481827	+	294	WP_003416635.1	hypothetical protein	-
GS11_RS16385	3482115..3483404	+	1290	WP_003416640.1	ATP-binding protein	-
GS11_RS16390	3483751..3484185	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
GS11_RS16395	3484188..3484640	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GS11_RS16400	3484871..3485215	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GS11_RS16405	3485212..3485541	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
GS11_RS16410	3486079..3486426	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
GS11_RS16415	3486423..3487043	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
GS11_RS16420	3487203..3488468	-	1266	WP_003909837.1	hypothetical protein	-
GS11_RS16425	3488636..3488845	+	210	WP_003416778.1	hypothetical protein	-
GS11_RS16435	3489092..3490126	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T47397 WP_003899954.1 NZ_CP008744:3484871-3485215 [Mycobacterium tuberculosis variant bovis BCG]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T47397 NZ_CP008744:3484871-3485215 [Mycobacterium tuberculosis variant bovis BCG]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT47397 WP_003899955.1 NZ_CP008744:3485212-3485541 [Mycobacterium tuberculosis variant bovis BCG]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT47397 NZ_CP008744:3485212-3485541 [Mycobacterium tuberculosis variant bovis BCG]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6