Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2184022..2184890 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag GS11_RS10140 Protein ID WP_010886136.1
Coordinates 2184022..2184399 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag GS11_RS10145 Protein ID WP_003409886.1
Coordinates 2184441..2184890 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS10090 2179811..2180263 - 453 WP_010950636.1 lipoprotein -
GS11_RS10095 2180327..2180728 + 402 WP_003409869.1 hypothetical protein -
GS11_RS10100 2180721..2180903 - 183 WP_003409870.1 hypothetical protein -
GS11_RS10105 2181017..2181367 - 351 WP_003409871.1 hypothetical protein -
GS11_RS10110 2181378..2182280 - 903 WP_003409874.1 hypothetical protein -
GS11_RS10115 2182301..2182492 - 192 WP_003409876.1 hypothetical protein -
GS11_RS10120 2182493..2182789 - 297 WP_003409877.1 hypothetical protein -
GS11_RS10125 2183029..2183244 + 216 WP_003409878.1 antitoxin -
GS11_RS10130 2183241..2183552 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
GS11_RS22325 2183526..2184047 - 522 WP_010950637.1 hypothetical protein -
GS11_RS10140 2184022..2184399 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
GS11_RS10145 2184441..2184890 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
GS11_RS10150 2184887..2185432 + 546 WP_003409891.1 SecB-like chaperone -
GS11_RS10155 2185321..2185935 - 615 WP_003901296.1 hypothetical protein -
GS11_RS10160 2185984..2186280 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
GS11_RS10165 2186277..2186528 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
GS11_RS10170 2186515..2187009 + 495 WP_003899099.1 hypothetical protein -
GS11_RS10175 2187169..2187576 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
GS11_RS10180 2187580..2187852 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GS11_RS10185 2187885..2189105 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T47375 WP_010886136.1 NZ_CP008744:2184022-2184399 [Mycobacterium tuberculosis variant bovis BCG]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T47375 NZ_CP008744:2184022-2184399 [Mycobacterium tuberculosis variant bovis BCG]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT47375 WP_003409886.1 NZ_CP008744:2184441-2184890 [Mycobacterium tuberculosis variant bovis BCG]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT47375 NZ_CP008744:2184441-2184890 [Mycobacterium tuberculosis variant bovis BCG]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References