Detailed information of TA system TA047299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1330726..1331321
• Replicon: chromosome
• Accession: NZ_CP008726
• Organism: Burkholderia oklahomensis strain EO147

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: DM82_RS05990
• Protein ID: WP_010111175.1
• Coordinates: 1331043..1331321 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DM82_RS05985
• Protein ID: WP_010111178.1
• Coordinates: 1330726..1331025 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM82_RS05950	1326420..1326995	+	576	WP_010111191.1	hypothetical protein	-
DM82_RS05955	1326995..1327309	+	315	WP_010111189.1	hypothetical protein	-
DM82_RS35920	1327372..1327791	+	420	WP_144444657.1	hypothetical protein	-
DM82_RS05960	1327791..1328135	+	345	WP_144444658.1	hypothetical protein	-
DM82_RS35925	1328122..1328532	+	411	WP_010111186.1	hypothetical protein	-
DM82_RS05970	1328576..1329544	+	969	WP_010111184.1	hypothetical protein	-
DM82_RS05975	1329541..1329918	-	378	WP_010111182.1	hypothetical protein	-
DM82_RS05980	1329922..1330660	+	739	Protein_1192	hypothetical protein	-
DM82_RS05985	1330726..1331025	-	300	WP_010111178.1	HigA family addiction module antidote protein	Antitoxin
DM82_RS05990	1331043..1331321	-	279	WP_010111175.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DM82_RS05995	1331383..1331736	+	354	WP_010111173.1	hypothetical protein	-
DM82_RS06000	1331733..1332914	+	1182	WP_010111171.1	baseplate J/gp47 family protein	-
DM82_RS06005	1332914..1333576	+	663	WP_010111169.1	DUF2612 domain-containing protein	-
DM82_RS06015	1334363..1335205	-	843	WP_038802590.1	IS3 family transposase	-
DM82_RS06020	1335323..1335763	+	441	WP_081464416.1	hypothetical protein	-
DM82_RS06025	1335787..1336110	+	324	WP_158332682.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1257185..1347667	90482

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10677.16 Da
• Isoelectric Point: 10.2114

>T47299 WP_010111175.1 NZ_CP008726:c1331321-1331043 [Burkholderia oklahomensis]
MITSFNCRDSEALFNGRRVARFANIERVAIRKLQQLHAAADLDFLRIPPNNRLEALKGDRQGQFSIRINDQWRICFRFAG
GRASDVEIVDYH

Nucleotide

• Length: 279 bp

>T47299 NZ_CP008726:c1331321-1331043 [Burkholderia oklahomensis]
ATGATTACATCGTTCAACTGCCGAGATTCGGAAGCGCTATTCAACGGCCGGCGGGTTGCTCGCTTTGCCAACATCGAGCG
CGTAGCTATCCGCAAGCTCCAGCAGTTGCATGCTGCGGCCGATCTAGACTTCCTCCGCATCCCGCCGAACAACCGGCTCG
AAGCACTGAAGGGAGACCGGCAAGGCCAATTCAGCATCCGCATCAACGATCAGTGGCGCATTTGCTTCAGGTTCGCTGGA
GGCCGCGCCTCTGATGTGGAAATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10953.55 Da
• Isoelectric Point: 5.6088

>AT47299 WP_010111178.1 NZ_CP008726:c1331025-1330726 [Burkholderia oklahomensis]
MTREVPLATPGEILALDWLEPMGISQYALAKAISVPARRINEIVKGDRAITADTAVRLGAFFGVDPQSWMNLQTHYDTAL
AKEKIGDDVLREIREHAHA

Nucleotide

• Length: 300 bp

>AT47299 NZ_CP008726:c1331025-1330726 [Burkholderia oklahomensis]
ATGACCCGAGAAGTACCCCTGGCGACGCCCGGTGAAATCCTGGCGCTCGACTGGCTGGAGCCGATGGGCATCAGCCAATA
CGCCCTCGCAAAAGCGATCAGTGTTCCGGCCCGCCGCATCAACGAAATCGTCAAAGGCGACCGTGCCATTACTGCAGATA
CGGCCGTGCGCCTCGGCGCGTTCTTCGGCGTGGATCCGCAGAGCTGGATGAATCTCCAGACGCACTACGACACCGCACTC
GCCAAGGAGAAAATTGGCGATGACGTGCTGCGCGAGATCCGAGAGCACGCGCACGCCTGA