Detailed information of TA system TA047288

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69703..69942
• Replicon: plasmid pEC648_1
• Accession: NZ_CP008715
• Organism: Escherichia coli strain ST648 isolate Pleural Effusion of Patients with Empyema Thoracis

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: FH07_RS25355
• Protein ID: WP_023144756.1
• Coordinates: 69808..69942 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	69703..69763 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH07_RS25335	67232..67978	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
FH07_RS25340	68033..68593	+	561	WP_000139341.1	fertility inhibition protein FinO	-
FH07_RS25345	68725..68937	+	213	WP_013023861.1	hypothetical protein	-
FH07_RS25350	69450..69736	+	287	Protein_76	DUF2726 domain-containing protein	-
-	69703..69763	-	61	NuclAT_0	-	Antitoxin
-	69703..69763	-	61	NuclAT_0	-	Antitoxin
-	69703..69763	-	61	NuclAT_0	-	Antitoxin
-	69703..69763	-	61	NuclAT_0	-	Antitoxin
FH07_RS25355	69808..69942	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
FH07_RS27335	70239..70349	+	111	Protein_78	replication protein	-
FH07_RS25360	70418..71200	-	783	WP_001300609.1	IS21-like element IS100kyp family helper ATPase IstB	-
FH07_RS25365	71197..72219	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
FH07_RS27340	72300..72452	+	153	WP_072258247.1	replication protein A	-
FH07_RS29035	72558..72692	+	135	Protein_82	protein CopA/IncA	-
FH07_RS25370	72689..72763	+	75	WP_031943482.1	RepA leader peptide Tap	-
FH07_RS25375	72756..73613	+	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B / aadA5 / qacE / sul1 / mph(A) / tet(B)	iucA / iucB / iucC / iucD / iutA	1..120653	120653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T47288 WP_023144756.1 NZ_CP008715:69808-69942 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T47288 NZ_CP008715:69808-69942 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT47288 NZ_CP008715:c69763-69703 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin