Detailed information of TA system TA047250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4341265..4342064
• Replicon: chromosome
• Accession: NZ_CP008697
• Organism: Escherichia coli strain ST648 isolate Pleural Effusion of Patients with Empyema Thoracis

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: FH07_RS21435
• Protein ID: WP_000347273.1
• Coordinates: 4341600..4342064 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: FH07_RS21430
• Protein ID: WP_001308975.1
• Coordinates: 4341265..4341600 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH07_RS21415	4337050..4337820	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
FH07_RS21420	4337836..4339170	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
FH07_RS21425	4339545..4341116	+	1572	WP_023908830.1	galactarate dehydratase	-
FH07_RS21430	4341265..4341600	+	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
FH07_RS21435	4341600..4342064	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
FH07_RS21440	4342119..4342928	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
FH07_RS21445	4343177..4344457	+	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
FH07_RS21450	4344480..4344953	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
FH07_RS21455	4344964..4345743	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
FH07_RS21460	4345733..4346611	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
FH07_RS21465	4346629..4347063	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T47250 WP_000347273.1 NZ_CP008697:4341600-4342064 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T47250 NZ_CP008697:4341600-4342064 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT47250 WP_001308975.1 NZ_CP008697:4341265-4341600 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT47250 NZ_CP008697:4341265-4341600 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08