Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2972051..2972553 Replicon chromosome
Accession NZ_CP008697
Organism Escherichia coli strain ST648 isolate Pleural Effusion of Patients with Empyema Thoracis

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FH07_RS14830 Protein ID WP_000767829.1
Coordinates 2972051..2972305 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FH07_RS14835 Protein ID WP_001259255.1
Coordinates 2972302..2972553 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH07_RS14805 2967066..2967293 - 228 WP_000234896.1 sulfurtransferase TusA -
FH07_RS14810 2967307..2968365 - 1059 WP_000492339.1 transport protein YeeE -
FH07_RS14815 2968544..2969902 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FH07_RS28500 2969892..2969954 - 63 WP_010723108.1 membrane protein YoeI -
FH07_RS14820 2970169..2971098 - 930 WP_000803351.1 LysR family transcriptional regulator -
FH07_RS14825 2971144..2971968 - 825 WP_000754737.1 SDR family oxidoreductase -
FH07_RS14830 2972051..2972305 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FH07_RS14835 2972302..2972553 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FH07_RS26680 2972836..2972886 + 51 WP_001364200.1 his operon leader peptide -
FH07_RS14840 2973032..2973931 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FH07_RS14845 2973937..2975235 + 1299 WP_000009595.1 histidinol dehydrogenase -
FH07_RS14850 2975238..2976308 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
FH07_RS14855 2976308..2977375 + 1068 WP_000080135.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T47246 WP_000767829.1 NZ_CP008697:c2972305-2972051 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T47246 NZ_CP008697:c2972305-2972051 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT47246 WP_001259255.1 NZ_CP008697:c2972553-2972302 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT47246 NZ_CP008697:c2972553-2972302 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References