Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1065094..1065788 Replicon chromosome
Accession NZ_CP008697
Organism Escherichia coli strain ST648 isolate Pleural Effusion of Patients with Empyema Thoracis

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag FH07_RS05340 Protein ID WP_001263491.1
Coordinates 1065390..1065788 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag FH07_RS05335 Protein ID WP_000554755.1
Coordinates 1065094..1065387 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH07_RS05310 1060129..1060389 - 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -
FH07_RS05315 1060575..1061348 + 774 WP_022645219.1 C40 family peptidase -
FH07_RS05320 1061446..1063158 - 1713 Protein_1006 flagellar biosynthesis protein FlhA -
FH07_RS05325 1063130..1063915 + 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
FH07_RS05330 1063986..1065042 + 1057 Protein_1008 DNA polymerase IV -
FH07_RS05335 1065094..1065387 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FH07_RS05340 1065390..1065788 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FH07_RS05345 1065798..1066251 + 454 Protein_1011 GNAT family N-acetyltransferase -
FH07_RS05350 1066442..1067581 + 1140 WP_022645224.1 RNA ligase RtcB family protein -
FH07_RS05355 1067578..1068192 + 615 WP_022645225.1 peptide chain release factor H -
FH07_RS05360 1068254..1068763 + 510 WP_001361775.1 hydrolase -
FH07_RS05365 1068780..1069061 - 282 WP_022645226.1 hypothetical protein -
FH07_RS05370 1069070..1070527 - 1458 WP_022645227.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T47232 WP_001263491.1 NZ_CP008697:1065390-1065788 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T47232 NZ_CP008697:1065390-1065788 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT47232 WP_000554755.1 NZ_CP008697:1065094-1065387 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT47232 NZ_CP008697:1065094-1065387 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References