Detailed information of TA system TA047225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 174136..174738
• Replicon: chromosome
• Accession: NZ_CP008697
• Organism: Escherichia coli strain ST648 isolate Pleural Effusion of Patients with Empyema Thoracis

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FH07_RS00865
• Protein ID: WP_000897305.1
• Coordinates: 174136..174447 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FH07_RS00870
• Protein ID: WP_000356397.1
• Coordinates: 174448..174738 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH07_RS00835	169166..169951	+	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-
FH07_RS00840	170050..170649	+	600	WP_001314324.1	glucose-1-phosphatase	-
FH07_RS00845	170643..171515	+	873	WP_000920762.1	virulence factor BrkB family protein	-
FH07_RS00850	171512..171949	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FH07_RS00855	171994..172935	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FH07_RS00860	172999..173907	-	909	WP_001318161.1	alpha/beta hydrolase	-
FH07_RS00865	174136..174447	+	312	WP_000897305.1	hypothetical protein	Toxin
FH07_RS00870	174448..174738	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FH07_RS00880	175343..175561	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
FH07_RS00885	175776..176705	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
FH07_RS00890	176702..177337	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FH07_RS00895	177334..178236	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T47225 WP_000897305.1 NZ_CP008697:174136-174447 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T47225 NZ_CP008697:174136-174447 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT47225 WP_000356397.1 NZ_CP008697:174448-174738 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT47225 NZ_CP008697:174448-174738 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7