Detailed information of TA system TA047222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4334461..4335115
• Replicon: chromosome
• Accession: NZ_CP008696
• Organism: Pseudomonas chlororaphis strain PA23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EY04_RS19160
• Protein ID: WP_038636757.1
• Coordinates: 4334461..4334811 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EY04_RS19165
• Protein ID: WP_009049793.1
• Coordinates: 4334801..4335115 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EY04_RS19150	4331211..4332743	+	1533	WP_009049790.1	NADH-quinone oxidoreductase subunit M	-
EY04_RS19155	4332751..4334214	+	1464	WP_007929613.1	NADH-quinone oxidoreductase subunit NuoN	-
EY04_RS19160	4334461..4334811	+	351	WP_038636757.1	toxin	Toxin
EY04_RS19165	4334801..4335115	+	315	WP_009049793.1	transcriptional regulator	Antitoxin
EY04_RS19170	4335281..4337023	-	1743	WP_023964376.1	ABC transporter substrate-binding protein	-
EY04_RS19175	4337187..4337459	-	273	WP_009049795.1	DUF2160 domain-containing protein	-
EY04_RS19180	4337470..4338270	-	801	WP_007929618.1	carbohydrate ABC transporter permease	-
EY04_RS19185	4338282..4339148	-	867	WP_016701903.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13987.96 Da
• Isoelectric Point: 9.4183

>T47222 WP_038636757.1 NZ_CP008696:4334461-4334811 [Pseudomonas chlororaphis]
MGALFIELPPFQRHRQDYLDDELFRNLQLELLKAPEAGDLIERTDGLRKIRFVDERRHKGKRGGIRVIYYWWSGDAQFWL
FTLYAKNEQNDLTPHQKKLLKQLLNREVEARTHHET

Nucleotide

• Length: 351 bp

>T47222 NZ_CP008696:4334461-4334811 [Pseudomonas chlororaphis]
ATGGGCGCACTTTTTATTGAGCTGCCCCCGTTCCAACGCCATCGACAGGACTACCTGGACGACGAGCTTTTTCGTAATTT
GCAGCTTGAGTTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGAGAACCGATGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTATTACTGGTGGTCCGGAGACGCCCAATTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACACCCCATCAGAAAAAACTGCTCAAGCAACTGCTGAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11917.85 Da
• Isoelectric Point: 10.6052

>AT47222 WP_009049793.1 NZ_CP008696:4334801-4335115 [Pseudomonas chlororaphis]
MKRDIFSELVEGFDALAKERQGKLTLRTYKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRTNTRTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLAALA

Nucleotide

• Length: 315 bp

>AT47222 NZ_CP008696:4334801-4335115 [Pseudomonas chlororaphis]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTGCG
CACCTACAAGGTCAAGCTGAACGCGCTGCTCCCGATCACCGCTGAAGAAGTGCTGGCCGTGCGCGAGCAGTTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCACCAATACTCGGACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCTCAGGCCACCACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCACTGGCCTGA