Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3540676..3541346 Replicon chromosome
Accession NZ_CP007809
Organism Mycobacterium tuberculosis strain KIT87190

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag ER17_RS16760 Protein ID WP_003899954.1
Coordinates 3540676..3541020 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag ER17_RS16765 Protein ID WP_003899955.1
Coordinates 3541017..3541346 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ER17_RS16730 3535749..3536609 + 861 WP_003416628.1 alpha/beta hydrolase -
ER17_RS16735 3536584..3537099 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
ER17_RS16740 3537339..3537632 + 294 WP_003416635.1 hypothetical protein -
ER17_RS16745 3537920..3539209 + 1290 WP_003416640.1 ATP-binding protein -
ER17_RS16750 3539556..3539990 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
ER17_RS16755 3539993..3540445 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ER17_RS16760 3540676..3541020 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ER17_RS16765 3541017..3541346 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
ER17_RS16770 3541884..3542231 + 348 WP_003899956.1 DUF2384 domain-containing protein -
ER17_RS16775 3542228..3542848 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
ER17_RS16780 3543008..3544273 - 1266 WP_003902423.1 hypothetical protein -
ER17_RS16785 3544441..3544650 + 210 WP_003416778.1 hypothetical protein -
ER17_RS16795 3544897..3545931 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T47206 WP_003899954.1 NZ_CP007809:3540676-3541020 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T47206 NZ_CP007809:3540676-3541020 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT47206 WP_003899955.1 NZ_CP007809:3541017-3541346 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT47206 NZ_CP007809:3541017-3541346 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References