Detailed information of TA system TA047148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 944761..945400
• Replicon: chromosome
• Accession: NZ_CP007805
• Organism: Haemophilus influenzae CGSHiCZ412602

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: H733_RS04515
• Protein ID: WP_005650215.1
• Coordinates: 944761..945066 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H733_RS04520
• Protein ID: WP_005650217.1
• Coordinates: 945077..945400 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H733_RS04495	940191..942230	-	2040	WP_038439719.1	excinuclease ABC subunit B	-
H733_RS04505	942845..943813	-	969	WP_014550701.1	zinc transporter permease subunit ZevB	-
H733_RS04510	943816..944436	-	621	WP_038439720.1	zinc transporter binding subunit ZevA	-
H733_RS04515	944761..945066	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H733_RS04520	945077..945400	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
H733_RS04525	945563..947233	+	1671	WP_005663485.1	energy-dependent translational throttle protein EttA	-
H733_RS04530	947295..947636	+	342	WP_038439721.1	SirB2 family protein	-
H733_RS04535	947641..949485	+	1845	WP_038439722.1	tRNA(Met) cytidine acetyltransferase TmcA	-
H733_RS09070	949482..949655	+	174	WP_005652999.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T47148 WP_005650215.1 NZ_CP007805:944761-945066 [Haemophilus influenzae CGSHiCZ412602]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T47148 NZ_CP007805:944761-945066 [Haemophilus influenzae CGSHiCZ412602]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGAGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT47148 WP_005650217.1 NZ_CP007805:945077-945400 [Haemophilus influenzae CGSHiCZ412602]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT47148 NZ_CP007805:945077-945400 [Haemophilus influenzae CGSHiCZ412602]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACACTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAACCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70